The HBA Test Kit is a qualitative kit for detecting of 3 mutations in HBA gene, including c.369C>G, c.377T>C, c.427T>C. The kit is for use on DNA samples extracted from human peripheral blood and will provide a qualitative assessment of mutation status. The results obtained from the test aids researchers in determining correlations between mutations and thalassemia.

Principle

The test is based on the technology of multicolor melting curve analysis (MMCA). Mutations are identified by the different Tm values of hybridizing PCR products with the fluorescent probes. According to this design, 3 mutations of HBA gene can be accurately detected in a single reaction with HBA PCR Mix.

For Research Use Only