The Del-HBA-T Test Kit is a qualitative kit for detecting of 4 deletions in human α-globin gene (HBA), including –^SEA, -α^4.2, –^THAI and -α^3.7. The kit is for use on DNA samples extracted from human peripheral blood and will provide a qualitative assessment of mutation status. The results obtained from the test aids researchers in determining correlations between mutations and thalassemia.

Principle

The test is based on the technology of multi-color melting curve analysis (MMCA). Mutations are identified by the different Tm values of hybridizing PCR products with the fluorescent probes. According to this design, 4 deletions of HBA gene can be accurately detected in two reactions with Del-HBA-T PCR Mix A and Del-HBA-T PCR Mix B.

For Research Use Only