PML-RARα V Detection Kit is intended to quantify the PML-RARα V transcripts in human bone marrow or whole blood samples of leukemia patients. The results obtained from the kit provides information about efficiency of treatment or Minimal Residual Disease (MRD) in patients.

Principle

PML-RARα V Detection Kit is a RT-qPCR based assay for quantification of PML-RARα V transcripts. The kit contains RT reaction mix and qPCR mix. cDNA is synthesized by adding purified total RNA to the RT reaction mix. The resulting cDNA is added to 2 PCR reaction tubes, which contain specific PCR primers and probes for PML-RARα V and ABL1. The qPCR is performed in a real-time thermal cycler with optical filters for FAM dye. After PCR, a linear regression curve is calculated from the standards for each gene and used for calculating the copy number of each gene in an unknown sample. PML-RARα V transcripts levels will be expressed as percentage of PML-RARα V over ABL1.

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