Manual MTB DNA Extraction Kit is designed for isolation and purification of nucleic acids from sputum sample, tuberculosis cultured sample and bronchial washing fluid, bronchoalveolar lavage fluid (BALF) sample. The product provides high-quality nucleic acids for downstream applications, such as amplification or other enzymatic reactions. The product is intended to be used by professional users, such as technicians and physicians who are trained in molecular biological techniques.

This kit can used with Zeesan TB/Drug-resistant TB Detection kit for the MTB and DR-TB detection.

Principle

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, comprising four steps: lysis, binding, washing, and elution.

Lysis: The samples are lysed first in the presence of Lysis Buffer, which allows the nucleic acids to be released.
Binding: Binding buffer is added to the lysed samples to adjust binding conditions. Lysates are thoroughly mixed with magnetic particles to allow optimal adsorption of nucleic acids to the silica surface. Salt and pH conditions ensure that protein and other contaminants, which can inhibit PCR and other downstream enzymatic reactions, are not bound to the magnetic particles.
Washing: While the nucleic acids remain bound to the magnetic particles, contaminants are efficiently washed away during a sequence of wash steps.
Elution: Highly pure nucleic acids is eluted in Elution Buffer. The purified nucleic acids can be either used immediately in downstream applications or stored for future use.

Sample Type

Sputum, tuberculosis culture, bronchial washing fluid, bronchoalveolar lavage fluid (BALF)

Order Information

For research use only