The MTB/PZA Test Kit (MMCA)(Abbreviation: MTB/PZA Test Kit) is an in vitro diagnostic reagent used for qualitative detection of pyrazinamide-resistance mutations in Mycobacterium tuberculosis from cultured Mycobacterium tuberculosis specimens or sputum samples. The kit employs PCR and melting curve analysis methods, and covers the mutations of pncA (561 bp) and pncA promoter region (positions -16~-1) to determine the resistance of pyrazinamide.

Principle

The MTB/PZA Test Kit employs dually labeled probes to target the excess single-stranded amplicons generated by asymmetric PCR; then uses melting curve analysis to generate the minus derivative of the fluorescence intensity versus temperature, and compares the melting temperature (Tm) to gain mutation information of the sequences. Where, perfect match of target sequence and probe gives the highest Tm; partially mismatch, e.g., point mutation, insertion or deletion, would cause decreased Tm than that of perfect match. The decreased degree is related to the type of point mutation, base number of insertion or deletion as well as the mutation location.

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