The BCR-ABL1 Genotyping Kit is a qualitative test for differentiating three splice variants of BCR-ABL1 associated with leukemia: BCR-ABL1 p210, BCR-ABL1 p190, and BCR-ABL1 p230. The kit detects RNA transcripts of fusion genes extracted from human bone marrow or whole blood samples with BCR-ABL1.

Principle

BCR-ABL1 Genotyping Kit is a RT-qPCR based assay for differentiating the subtype of BCR-ABL1 p210, BCR-ABL1 p190, BCR-ABL1 p230. Included in the kit are RT reaction mix and qPCR mix. cDNA is synthesized by adding purified total RNA to the RT reaction mix. The resulting cDNA is added to qPCR reaction tube, which contain specific PCR primers and probes for detection of fusion genes and an internal control gene of GUSB. The qPCR is performed in a real-time thermal cycler with optical filters for the detection of FAM, HEX, ROX and Cy5 fluorescence signals. Amplification plots and the resulting Cq (Quantification cycle) values are used for the identification of the fusion gene subtype. Detection of the GUSB gene is an internal control for the integrity of the RNA sample and functionality of both cDNA and qPCR reactions.

For Research Use Only